Original Articles

Effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose} on human hepatocellular carcinoma cell line

Authors: Jing Wu, Wei Kou, Ming-tai Gao, Yong-ning Zhou, Ai-qin Wang, Qun-ji Xue, Liang Qiao


Aim: To study the effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose (COPADG) on cultured human hepatocellular carcinoma cells (HepG2).
Methods: HepG2 cells were cultured in RPMI-1640 medium. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry.
Results: At the concentration ranging between 1–30 mumol/L, COPADG potently inhibited the growth and induced apoptosis of HepG2 cells.
Conclusion: COPADG could effectively induce apoptosis in human hepatocellular carcinoma cells. More investigations are warranted for the potential use of this compound as a new agent for the non-surgical management of human hepatocellular carcinoma.

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