Inhibition of myocardial inward rectifier potassium current by propylbutyldopamine
Abstract
AIM:
To study the effects of propylbutyldopamine (PBDA) on the inward rectifier potassium current (Ik1).
METHODS:
The quasi-steady state current-voltage relationship from the isolated guinea pig ventricular cells were measured using whole-cell patch-clamp techniques with a slow ramp depolarization (8 mV.s-1).
RESULTS:
PBDA 5, 50, and 100 mumol.L-1 concentration-dependently reduced the inward rectifier potassium current. PBDA blocked Ik1 in guinea pig ventricular cells. The effect of PBDA was not blocked by the selective dopamine D2-receptor blocker, domperidone.
CONCLUSION:
PBDA inhibited Ik1 directly, independent of the dopamine D2-receptor.
Keywords:
To study the effects of propylbutyldopamine (PBDA) on the inward rectifier potassium current (Ik1).
METHODS:
The quasi-steady state current-voltage relationship from the isolated guinea pig ventricular cells were measured using whole-cell patch-clamp techniques with a slow ramp depolarization (8 mV.s-1).
RESULTS:
PBDA 5, 50, and 100 mumol.L-1 concentration-dependently reduced the inward rectifier potassium current. PBDA blocked Ik1 in guinea pig ventricular cells. The effect of PBDA was not blocked by the selective dopamine D2-receptor blocker, domperidone.
CONCLUSION:
PBDA inhibited Ik1 directly, independent of the dopamine D2-receptor.