Genetic effects of fluorocarbon emulsion on germinal cells of male mice
Abstract
The A strain male mice were infused with fluorocarbon emulsion (FCE) 50, 10 ml/kg and NS 50 ml/kg. Metaphase plates of spermatogonia and spermatocytes I were examined on d 7 after infusion and histological damage of the testes was studied by light and electron microscopes.
The frequencies of aneuploid and polyploidy of spermatogonia were 2.9, 2.5, and 1.4%, respectively induced by FCE 50, 10 ml/kg and NS 50 ml/kg. While those of breaks and reciprocal translocations were 0.5, 0.1 and 0.2%, respectively. In spermatocytes I the frequency of X-Y univalent and autosomal univalent were 2% (P<0.05) and no morphological changes were found in spermatogonia developed from spermatogenic cells.
It may be concluded that no chromosomal aberrations were induced in germinal cells of male mice by FCE.
Keywords:
The frequencies of aneuploid and polyploidy of spermatogonia were 2.9, 2.5, and 1.4%, respectively induced by FCE 50, 10 ml/kg and NS 50 ml/kg. While those of breaks and reciprocal translocations were 0.5, 0.1 and 0.2%, respectively. In spermatocytes I the frequency of X-Y univalent and autosomal univalent were 2% (P<0.05) and no morphological changes were found in spermatogonia developed from spermatogenic cells.
It may be concluded that no chromosomal aberrations were induced in germinal cells of male mice by FCE.