Co-activation of nAChR and mGluR induces γ oscillation in rat medial septum diagonal band of Broca slices
Abstract
Ya-li WANG1, Jian-gang WANG2, Gao-xiang OU-YANG3, Xiao-li LI3, Zaineb HENDERSON4, Cheng-biao LU1, *
1Department of Physiology and Neurobiology, Henan province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003, China; 2Department of pathophysiology, Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003, China; 3National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China; 4Insitute of Membrane and System biology, University of Leeds, Leeds, UK
Aim: To examine whether co-activation of nAChR and mGluR1 induced γ oscillation (20–60 Hz) in rat medial septum diagonal band of Broca (MSDB) slices.
Methods: Rat brain sagittal slices containing the MSDB were prepared. Extracellular field potentials were recorded with glass microelectrodes. The nAChR and mGluR1 agonists were applied to the slices to induce network activity. Data analysis was performed off-line using software Spike 2.
Results: Co-application of the nAChR agonist nicotine (1 µmol/L) and the mGluR1 agonist dihydroxyphenylglycine (DHPG, 25 µmol/L) was able to induce γ oscillation in MSDB slices. The intensity of nAChR and mGluR1 activation was critical for induction of network oscillation at a low (θ oscillation) or high frequency (γ oscillation): co-application of low concentrations of the two agonists only increased the power and frequency of oscillation within the range of θ, whereas γ oscillation mostly appeared when high concentrations of the two agonists were applied.
Conclusion: Activation of mGluR1 and nAChR is able to program slow or fast network oscillation by altering the intensity of receptor activation, which may provide a mechanism for modulation of learning and memory.
Keywords: nAChR; mGluR1; DHPG; nicotine; θ oscillation; γ oscillation; the medial septum diagonal band of Broca; learning and memory
This work was supported by the National Natural Science Foundation of China (No 31070938; 81271422); the open project of the National Key Laboratory of Cognitive Neuroscience and Learning in Beijing Normal University and National Natural Science Funds for Distinguished Young Scholar (No 61025019); International Science and Technology Cooperation Projects of Henan Province (2012); Scientific Reserch Fund of Xinxinag Medical University.
* To whom correspondence should be addressed.
E-mail Johnlu9001@hotmail.com
Received 2013-05-30 Accepted 2013-08-28
Keywords:
1Department of Physiology and Neurobiology, Henan province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003, China; 2Department of pathophysiology, Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003, China; 3National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China; 4Insitute of Membrane and System biology, University of Leeds, Leeds, UK
Aim: To examine whether co-activation of nAChR and mGluR1 induced γ oscillation (20–60 Hz) in rat medial septum diagonal band of Broca (MSDB) slices.
Methods: Rat brain sagittal slices containing the MSDB were prepared. Extracellular field potentials were recorded with glass microelectrodes. The nAChR and mGluR1 agonists were applied to the slices to induce network activity. Data analysis was performed off-line using software Spike 2.
Results: Co-application of the nAChR agonist nicotine (1 µmol/L) and the mGluR1 agonist dihydroxyphenylglycine (DHPG, 25 µmol/L) was able to induce γ oscillation in MSDB slices. The intensity of nAChR and mGluR1 activation was critical for induction of network oscillation at a low (θ oscillation) or high frequency (γ oscillation): co-application of low concentrations of the two agonists only increased the power and frequency of oscillation within the range of θ, whereas γ oscillation mostly appeared when high concentrations of the two agonists were applied.
Conclusion: Activation of mGluR1 and nAChR is able to program slow or fast network oscillation by altering the intensity of receptor activation, which may provide a mechanism for modulation of learning and memory.
Keywords: nAChR; mGluR1; DHPG; nicotine; θ oscillation; γ oscillation; the medial septum diagonal band of Broca; learning and memory
This work was supported by the National Natural Science Foundation of China (No 31070938; 81271422); the open project of the National Key Laboratory of Cognitive Neuroscience and Learning in Beijing Normal University and National Natural Science Funds for Distinguished Young Scholar (No 61025019); International Science and Technology Cooperation Projects of Henan Province (2012); Scientific Reserch Fund of Xinxinag Medical University.
* To whom correspondence should be addressed.
E-mail Johnlu9001@hotmail.com
Received 2013-05-30 Accepted 2013-08-28