Effect of Misgurnus anguillicaudatus polysaccharide on immune responses of splenocytes in mice.
Abstract
AIM: To investigate the effect of Misgurnus anguillicaudatus polysaccharides
(MAP) on immune responses of splenocytes in mice.
METHODS: T lymphocyte proliferation (TLP) was measured by [3H]thymidine
incorporation assay. Cytotoxic T lymphocyte (CTL) cytotoxicity and natural killer
(NK) activity were determined by release of radioactive chromium [51Cr] from
pre-labeled target cells.
RESULTS: MAP 5 and 10 mg . kg-1 . d-1, ip for 7 d, could increase TLP, enhance
the cytotoxicity of CTL and NK cells, and antagonize the effect of concanavalin A
(ConA) on TLP suppressed by cyclophosphamide (CP). Inhibitory rates of CTL
cytotoxicity were decreased from 51.4 % in CP control mice to 18.2 % and 35.1 %
in MAP-treated CP mice, respectively. Furthermore, administration of MAP 10 and
20 mg . kg-1 . d-1, ip for 7 d, restored the reduced NK cell cytotoxicity caused
by CP administration in mice.
CONCLUSION: MAP has protective effect on augmenting T-cell-mediated immunity and
NK activity in normal and CP-treated mice.
Keywords:
(MAP) on immune responses of splenocytes in mice.
METHODS: T lymphocyte proliferation (TLP) was measured by [3H]thymidine
incorporation assay. Cytotoxic T lymphocyte (CTL) cytotoxicity and natural killer
(NK) activity were determined by release of radioactive chromium [51Cr] from
pre-labeled target cells.
RESULTS: MAP 5 and 10 mg . kg-1 . d-1, ip for 7 d, could increase TLP, enhance
the cytotoxicity of CTL and NK cells, and antagonize the effect of concanavalin A
(ConA) on TLP suppressed by cyclophosphamide (CP). Inhibitory rates of CTL
cytotoxicity were decreased from 51.4 % in CP control mice to 18.2 % and 35.1 %
in MAP-treated CP mice, respectively. Furthermore, administration of MAP 10 and
20 mg . kg-1 . d-1, ip for 7 d, restored the reduced NK cell cytotoxicity caused
by CP administration in mice.
CONCLUSION: MAP has protective effect on augmenting T-cell-mediated immunity and
NK activity in normal and CP-treated mice.