Original Article

Probucol inhibits oxidized-low density lipoprotein-induced adhesion of monocytes to endothelial cells in vitro.

Authors: Ge-Xiu LIU, Da-Ming OU, Li-Xin LI, Lin-Xi CHEN, Hong-Lin HUANG, Duan-Fang LIAO, Chao-Shu TANG

Abstract

AIM: To investigate the mechanism by which probucol (PBC) affected adhesion of
monocytes to human umbilical vein endothelial cells (HUVEC).
METHODS: Effects of PBC on expression of intercellular adhesion molecule-1
(ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, a nd E-selectin
in human umbilical vein endothelial cells were examined. Moreover, the inhibitory
effect of PBC were compared with that of monoclonal antibodies (mAbs) to ICAM-1,
VCAM-1, P-selectin, and E-select in on adhesion induced by oxidized-low density
lipoprotein(Ox-LDL).
RESULTS: PBC at 10 to 80 micromol/L inhibited Ox-LD L-induced adhesion index from
16.7 % to 7.0 % (P < 0.01) and Ox-LDL-induced expression of ICAM-1 (75 %) and
P-selectin (72 %). mAbs to ICAM -1 or P-selectin, when used alone, could only
slightly reduce the adhesion of monocyte to HUVEC. When both monoclonal
antibodies were used in combination, the adhesion was markedly inhibited from
16.7 % to 11.3 % (P < 0.01), but the effect was still weaker than that of PBC
(average 9.3 %).
CONCLUSION: PBC exerts its inhibitory effect on the adhesion of monocyte to HUVEC
by inhibiting the expression of ICAM-1 and P-selectin.
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