Effect of melatonin on production of hydroxyl radical and lactate dehydrogenase during hypoxia in rat cortical slices
Abstract
AIM:
To study the effect of melatonin on the production of hydroxyl radical (.OH) and lactate dehydrogenase (LDH) following hypoxia in cortical slice.
METHODS:
Cortical slice was incubated with artificial cerebrospinal fluid (ACSF) in tube. Hypoxia was achieved by substituting 91.6% N2 and 8.4% O2. The salicylate trapping method was used to measure hydroxyl radicals generated. The content of LDH in medium after hypoxia was measured by International Federation of Clinic Chemistry (IFCC) method.
RESULTS:
The contents of dihydroxybenzoic acid (DHBA) were increased significantly during hypoxia and reoxygenation in cortical slice. The production of DHBA in reoxygenation was decreased concentration-dependently by melatonin, but not during hypoxia 30 min. The release of LDH during hypoxia was steadily elevated and melatonin decreased the content of LDH after hypoxia.
CONCLUSION:
Melatonin decreased the injury and production of .OH after hypoxia.
Keywords:
To study the effect of melatonin on the production of hydroxyl radical (.OH) and lactate dehydrogenase (LDH) following hypoxia in cortical slice.
METHODS:
Cortical slice was incubated with artificial cerebrospinal fluid (ACSF) in tube. Hypoxia was achieved by substituting 91.6% N2 and 8.4% O2. The salicylate trapping method was used to measure hydroxyl radicals generated. The content of LDH in medium after hypoxia was measured by International Federation of Clinic Chemistry (IFCC) method.
RESULTS:
The contents of dihydroxybenzoic acid (DHBA) were increased significantly during hypoxia and reoxygenation in cortical slice. The production of DHBA in reoxygenation was decreased concentration-dependently by melatonin, but not during hypoxia 30 min. The release of LDH during hypoxia was steadily elevated and melatonin decreased the content of LDH after hypoxia.
CONCLUSION:
Melatonin decreased the injury and production of .OH after hypoxia.