Effect of recombinant human fibronectin polypeptide CH50 on growth and metastasis of melanoma
Abstract
AIM:
To study the effect of recombinant human fibronectin polypeptide CH50 on murine melanoma growth and metastasis, and its antitumor mechanism.
METHODS:
Mouse and melanoma B16 cell tests were used to observe antitumor effect and mechanism of CH50.
RESULTS:
CH50 markedly inhibited melanoma growth and experimental lung metastasis. The melanoma weight was reduced from (2.3 +/- 1.2) g of control group to (0.7 +/- 0.8) g of test group (P < 0.05). CH50 at 0.125, 0.25, 0.5 mg/mouse reduced melanoma lung metastatic colonies from 87 +/- 49 of control group to 34 +/- 6, 14 +/- 12, 4 +/- 2, respectively. CH50 adhered to melanoma B16 cells and inhibited adhesion of B16 cells to laminin. CH50 enhanced the cytotoxicity of macrophages to melanoma B16 cells.
CONCLUSION:
CH50 inhibited tumor growth and metastasis of murine melanoma. The antitumor effect of CH50 is related to its adhesion ability to melanoma B16 cells and enhancing macrophage cytotoxicity.
Keywords:
To study the effect of recombinant human fibronectin polypeptide CH50 on murine melanoma growth and metastasis, and its antitumor mechanism.
METHODS:
Mouse and melanoma B16 cell tests were used to observe antitumor effect and mechanism of CH50.
RESULTS:
CH50 markedly inhibited melanoma growth and experimental lung metastasis. The melanoma weight was reduced from (2.3 +/- 1.2) g of control group to (0.7 +/- 0.8) g of test group (P < 0.05). CH50 at 0.125, 0.25, 0.5 mg/mouse reduced melanoma lung metastatic colonies from 87 +/- 49 of control group to 34 +/- 6, 14 +/- 12, 4 +/- 2, respectively. CH50 adhered to melanoma B16 cells and inhibited adhesion of B16 cells to laminin. CH50 enhanced the cytotoxicity of macrophages to melanoma B16 cells.
CONCLUSION:
CH50 inhibited tumor growth and metastasis of murine melanoma. The antitumor effect of CH50 is related to its adhesion ability to melanoma B16 cells and enhancing macrophage cytotoxicity.