Original Article

Rhein lysinate increases the median survival time of SAMP10 mice: protective role in the kidney

Gang Hu, Jiang Liu, Yong-zhan Zhen, Rong Xu, Yu Qiao, Jie Wei, Ping Tu, Ya-jun Lin
DOI: 10.1038/aps.2012.177


Gang HU1, #, Jiang LIU2, #, Yong-zhan ZHEN3, Rong XU1, Yu QIAO1, Jie WEI1, Ping TU2, *, Ya-jun LIN1, *
1The Key Laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China; 2Department of Endocrinology, the Third Hospital of Nanchang City, Nanchang 330009, China; 3Hebei United University, Tangshan 063000, China

Aim: To investigate the protective effects of rhein lysinate (RHL), a major bioactive constituent of the rhizome of rhubarb (Rheum palmatum Linn or Rheum tanguticum Maxim), against kidney impairment in senescence-prone inbred strain 10 (SAMP10) mice.
Methods: SAMP10 mice were orally administered RHL (25 or 50 mg/kg) daily until 50% of the mice died. Senescence-resistant inbred strain 1 (SAMR1) mice administered no drug were taken as control. The kidneys were harvested after animal death, and examined morphologically and with immunochemical assays. The levels of MAD, SOD and GSH-px in the kidneys were measured with a photometric method. The expression of inflammatory factors and related proteins in the kidneys was analyzed using Western blotting.

Results: Treatment of SAMP10 mice with RHL had no effect on the body weight or phenotype. However, RHL significantly prolonged the median survival time of SAMP10 mice by approximately 25%, as compared to untreated SAMP10 mice. Compared SAMR1 mice, SAMP10 mice had a significantly lower level of SOD in the kidneys, but had no significant difference in the MDA or GSH-px levels. Treatment of SAMP10 mice with RHL significantly reduced the MAD level, and increased the SOD and GSH-px levels in the kidneys. Glomerulonephritis was observed in SAMP10 mice but not in SAMR1 mice. RHL decreased the incidence of glomerulonephritis, and significantly decreased the levels of TNF-α, IL-6, NF-κB, collagen types I and III in the kidneys.

Conclusion: Accelerated senescence is associated with glomerulonephritis in SAMP10 mice, and RHL prolongs their median survival time by reducing the severity of glomerulonephritis.

Keywords: rhein lysinate; SAMP10; aging; kidney; glomerulonephritis; superoxide dismutase; malondialdehyde; glutathione peroxidase; TNF-α; IL-6; NF-κB; collagen

This work was supported by a grant from the National Natural Science Foundation of China (81001439).
* To whom correspondence should be addressed.
E-mail linyajun2010@126.com (Ya-jun LIN); tuping8877@126.com (Ping TU)
Received 2012-10-06 Accepted 2012-11-25

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