Original Article

Effects of lidamycin on genomic DNA in human hepatoma BEL-7402 cells.

Authors: Qi-Yang HE, Bing JIANG, Dian-Dong LI

Abstract

AIM: To further study the effect of enediyne antibiotic lidamycin (C1027) on
genomic DNA in human hepatoma BEL-7402 cells.
METHODS: The DNA patterns were detected by agarose gel electrophoresis. The gene
damage was revealed by Southern hybridization. The DNA repair was determined by
neutral agarose gel electrophoresis. The lidamycin-cleaved sites were determined
with superhelix GEM plasmid.
RESULTS: The DNA ladder patterns were observed after the BEL-7402 cells were
treated with lidamycin 1 micromol/L for 15, 30, 60 min. The extrons and introns
of active N-ras gene in the BEL-7402 cells were easily damaged by lidamycin at
low concentrations, but no damage in the silent IL-2 gene was observed. It was
difficult to repair DNA breaks after the BEL-7402 cells were treated with
lidamycin. The cleaved sites of GEM plasmid treated with lidamycin were -OH HO-.
CONCLUSION: Lidamycin can significantly damage genomic DNA in the hepatoma
BEL-7402 cells. The results make it helpful to elucidate the molecular mechanism
of potent cytotoxicities of lidamycin to tumor cells.
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