Microdialysis sampling and microbore liquid chromatography with electrochemical detection for determination of serotonin in rat brain striatum
Abstract
AIM:
To develop a sensitive method for determination of serotonin in biological samples.
METHODS:
A combination of microdialysis sampling and microbore liquid chromatography with electrochemical detection (LCEC) was established.
RESULTS:
Changes of serotonin in fg or pg in microdialysates from brain striatum of the free moving rat were easily determined.
CONCLUSION:
This developed method was useful for living animal research. Serotonin level in corpus striatum healthy rats was quite stable.
Keywords:
To develop a sensitive method for determination of serotonin in biological samples.
METHODS:
A combination of microdialysis sampling and microbore liquid chromatography with electrochemical detection (LCEC) was established.
RESULTS:
Changes of serotonin in fg or pg in microdialysates from brain striatum of the free moving rat were easily determined.
CONCLUSION:
This developed method was useful for living animal research. Serotonin level in corpus striatum healthy rats was quite stable.