Effects of low-molecular-weight Rehmannia glutinosa polysaccharides on p53 gene expression
Abstract
AIM: To study the effect of low-molecular-weight Rehmannia glutinosa
polysaccharides (LRPS) on p53 gene expression.
METHODS: The level of p53 gene expression was determined by quantitative
polymerase chain reaction and autoradiography.
RESULTS: The levels of p53 mRNA in Lewis lung cancer tissue were 0.46, 1.52,
1.48, and 1.60 respectively after saline, LRPS 20 and 40 mg.kg-1, and
cyclophosphamide 10 mg.kg-1, which were the effective doses inhibiting the growth
of tumor tissue cells in vivo. LRPS markedly increased p53 gene expression.
CONCLUSION: LRPS effect on p53 gene expression is one of the mechanisms of
antitumor activity.
Keywords:
polysaccharides (LRPS) on p53 gene expression.
METHODS: The level of p53 gene expression was determined by quantitative
polymerase chain reaction and autoradiography.
RESULTS: The levels of p53 mRNA in Lewis lung cancer tissue were 0.46, 1.52,
1.48, and 1.60 respectively after saline, LRPS 20 and 40 mg.kg-1, and
cyclophosphamide 10 mg.kg-1, which were the effective doses inhibiting the growth
of tumor tissue cells in vivo. LRPS markedly increased p53 gene expression.
CONCLUSION: LRPS effect on p53 gene expression is one of the mechanisms of
antitumor activity.