Inhibition of harringtonine-induced apoptosis by tetradecanoylphorbol acetate in human leukemia HL-60 cells
Abstract
AIM: To study the changes of the apoptosis induced by camptothecin (Cam) or
harringtonine (Har) in human leukemia HL-60 cells after the cells were
preincubated with tetradecanoylphorbol acetate (TA).
METHODS: Chromatin condensation observation, flow cytometry, DNA agarose gel
electrophoresis, and Dot blot hybridization.
RESULTS: After the HL-60 cells were preincubated with TA 200 nmol.L-1 for 6 h,
the apoptosis induced by Har 0.1 mg.L-1 for 3 h was drastically inhibited, and
the apoptosis by Cam 0.2 mg.L-1 for 3 h was partly inhibited. On the other hand,
the expression level of c-myc gene in HL-60 cells decreased apparently after the
preincubation of TA.
CONCLUSION: TA preincubation inhibited the apoptosis induced by Har obviously or
by Cam partly in human leukemia HL-60 cells, and the expression of c-myc gene
decreased drastically in the preincubated cells, which might result in the
inhibition of apoptosis.
Keywords:
harringtonine (Har) in human leukemia HL-60 cells after the cells were
preincubated with tetradecanoylphorbol acetate (TA).
METHODS: Chromatin condensation observation, flow cytometry, DNA agarose gel
electrophoresis, and Dot blot hybridization.
RESULTS: After the HL-60 cells were preincubated with TA 200 nmol.L-1 for 6 h,
the apoptosis induced by Har 0.1 mg.L-1 for 3 h was drastically inhibited, and
the apoptosis by Cam 0.2 mg.L-1 for 3 h was partly inhibited. On the other hand,
the expression level of c-myc gene in HL-60 cells decreased apparently after the
preincubation of TA.
CONCLUSION: TA preincubation inhibited the apoptosis induced by Har obviously or
by Cam partly in human leukemia HL-60 cells, and the expression of c-myc gene
decreased drastically in the preincubated cells, which might result in the
inhibition of apoptosis.