Modification of an ion-paired high pressure liquid chromatography for ticarcillin in human serum
Abstract
AIM: To develop a new HPLC assay for the clinical pharmacokinetic study of ticarcillin in human.
METHODS: Reverse phase ion-paired HPLC was developed with a Novapak C18 column. The mobile phase consisted of 20% of acetonitrile and 80% of phosphate buffer 10 mmol.L-1 (pH 2.0-2.5) containing tetrapentylammonium bromide of 1 mmol.L-1. The analysis was monitored at 225 nm and the flow rate of the mobile phase was 0.8 mL.min-1. Cefoxitin was used as an internal standard. The sample volume was 200 microL of serum.
RESULTS: The assay was linear in the range of 2-80 mg.L-1. The intrarun coefficients of variation (CV) for a ticarcillin 5 mg.L-1 check sample was 4.2% (n = 10), and 2.7% for a 60 mg.L-1 check sample (n = 10), respectively. The inter-run CV (n = 6) were 1.6% (5 mg.L-1) and 2.3% (60 mg.L-1), respectively. The average recovery of the assay was 96.6%.
CONCLUSION: This assay was sensitive, precise, and accurate for evaluating the clinical pharmacokinetics of ticarcillin.
Keywords:
METHODS: Reverse phase ion-paired HPLC was developed with a Novapak C18 column. The mobile phase consisted of 20% of acetonitrile and 80% of phosphate buffer 10 mmol.L-1 (pH 2.0-2.5) containing tetrapentylammonium bromide of 1 mmol.L-1. The analysis was monitored at 225 nm and the flow rate of the mobile phase was 0.8 mL.min-1. Cefoxitin was used as an internal standard. The sample volume was 200 microL of serum.
RESULTS: The assay was linear in the range of 2-80 mg.L-1. The intrarun coefficients of variation (CV) for a ticarcillin 5 mg.L-1 check sample was 4.2% (n = 10), and 2.7% for a 60 mg.L-1 check sample (n = 10), respectively. The inter-run CV (n = 6) were 1.6% (5 mg.L-1) and 2.3% (60 mg.L-1), respectively. The average recovery of the assay was 96.6%.
CONCLUSION: This assay was sensitive, precise, and accurate for evaluating the clinical pharmacokinetics of ticarcillin.