Inhibitory effect of tetrahydropalmatine on calcium current in isolated cardiomyocyte of guinea pig.
Abstract
"AIM:
To study the effect of tetrahydropalmatine (THP) on calcium channels in ventricular single cells of guinea pig heart.
METHODS:
Patch-clamp technique (whole cell recording) was used to observe calcium current in ventricular myocytes.
RESULTS:
THP decreased ICa in ventricular myocytes with a dose and frequency-dependent manner. THP (0.1, 1, and 10 mumol.L-1) decreased ICa from 1.15 +/- 0.22, 0.91 +/- 0.18, and 1.60 +/- 0.42 nA (control) to 0.9 +/- 0.21 (P < 0.01), 0.56 +/- 0.21 (P < 0.01), and 0.83 +/- 0.21 nA (P < 0.05), respectively, number of cells is five in each group (n = 5), and the rates of the depression of ICa were 22%, 38%, and 48%, respectively. The effect was easily reduced by washing the cell with the Tyrode's solution. The current-voltage relation curve showed that the potential producing peak value of ICa was 0 mV at which THP had the most markedly inhibited action on ICa. When the stimulating frequency was changed, ICa varied in a frequency-dependent manner 5 min after THP was given, and the inhibition of THP was stronger at 2 Hz than that at 0.1 Hz.
CONCLUSION:
THP possessed a Ca2+ channel blocking effect.
"
Keywords:
To study the effect of tetrahydropalmatine (THP) on calcium channels in ventricular single cells of guinea pig heart.
METHODS:
Patch-clamp technique (whole cell recording) was used to observe calcium current in ventricular myocytes.
RESULTS:
THP decreased ICa in ventricular myocytes with a dose and frequency-dependent manner. THP (0.1, 1, and 10 mumol.L-1) decreased ICa from 1.15 +/- 0.22, 0.91 +/- 0.18, and 1.60 +/- 0.42 nA (control) to 0.9 +/- 0.21 (P < 0.01), 0.56 +/- 0.21 (P < 0.01), and 0.83 +/- 0.21 nA (P < 0.05), respectively, number of cells is five in each group (n = 5), and the rates of the depression of ICa were 22%, 38%, and 48%, respectively. The effect was easily reduced by washing the cell with the Tyrode's solution. The current-voltage relation curve showed that the potential producing peak value of ICa was 0 mV at which THP had the most markedly inhibited action on ICa. When the stimulating frequency was changed, ICa varied in a frequency-dependent manner 5 min after THP was given, and the inhibition of THP was stronger at 2 Hz than that at 0.1 Hz.
CONCLUSION:
THP possessed a Ca2+ channel blocking effect.
"