Original Article

Effects of captopril and enalaprilat on intracellular Ca2+ content in isolated cardiomyocytes from rats

Authors: Jun Wang, Lu Zhang, Jian-Hua Qi, Pei-Jin Zhang, Pei-Jing Wei, Pei-Kun Gu, Zheng-Jun Jin

Abstract

AIM: To study the effects of ACEI captopril (Cap) and enalaprilat (Ena) on intracellular Ca2+ concentration ([Ca2+]i) in cardiac myocytes isolated from SHR and WKY rats.
METHODS: Using fluorescent probe Fura 2-AM combined with computer image processing technique to measure [Ca2+]i.
RESULTS: Resting [Ca2+]i was higher in SHR cardiac myocytes (174 +/- 5 nmol.L-1) than that in WKY rat myocytes (148 +/- 15 nmol.L-1, P < 0.01). Cap and Ena decreased the resting [Ca2+]i in SHR myocytes (161 +/- 11 and 166 +/- 7 nmol.L-1, respectively, P < 0.05) but not in WKY rat myocytes (P > 0.05). Both drugs inhibited [Ca2+]i increment induced by KCI, NE, or Ang II in SHR and WKY rat myocytes except on KCI-induced [Ca2+]i increment in WKY rat myocytes (P > 0.05).
CONCLUSION: Cap and Ena had direct effects on pathological voltage-operated calcium channel in cardiac myocytes.
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