Original Article

Wuweizisu C from Schisandra chinensis decreases membrane potential in C6 glioma cells

Authors: Young-whan Choi, Kyeok Kim, Ji-yeong Jo, Hyo-lim Kim, You-jin Lee, Woo-jung Shin, Santosh J Sacket, Mijin Han, Dong-soon Im

Abstract

Aim: To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells.
Methods: The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells.
Results: Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl-4,4′-dimethoxy-5,6,5′,6′-dimethylene dioxybipheny 1-2,2′-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of Gi/o proteins, phospholipase C, and extracellular Na+ on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca2+ [Ca2+]i concentration, but decreased the ATP-indu-ced Ca2+ increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC12 neuronal cells.
Conclusion: Our results suggest that the decrease in the membrane potential and the modulation of [Ca2+]i concentration by wuweizisu C could be important action mechanisms of wuweizisu C.
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