Effects of delsoline on beating rate of cultured rat myocardial cells
Abstract
Cells from neonatal rat ventricular myocardium were cultured in Eagle MEM. After the cells were exposed to delsoline (DS) 0.5 and 0.75 mmol/L for 15 min, the beating rate increased by 7+/-7 and 10+/-9%, respectively. At 2.5 mmol/L, the beating rate decreased by 43+/-30%, while the amplitude of contraction was diminished. The increase in spontaneous frequency induced by DS 0.75 mmol/L was antagonized by propranolol 0.01 mmol/L. But propranolol per se did not alter the beating rate. The positive chronotropic responses of isoproterenol 1 micromol/L were reversed by DS 1 mmol/L.
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