Effects of 4 psychotropic drugs and 4 central neurotransmitters on lipid peroxidation in human erythrocyte membrane
Abstract
Lipid peroxidation of rbc membrane was measured by thiobarbituric assay for malondialdehyde (MDA), a product of lipid peroxidation. A small amout of MDA was formed after the incubation of rbc membrane at 37 degreees. NE enhanced MDA formation at 20 nM-2 microM when NE was added to the incubated rbc membrane, but inhibited MDA formation at 2-200 microM. DA, 5-HT, and GABA showed promoting effects on MDA formation of rbc membrane at 200 nM; NE>=DA>5-HT>GABA.
Tricyclic antidepressants amitriptyline (AMT) and imipramine (IMP) accelerated MDA formation at 20 nM-20 microM whenAMT and IMP was added to rbc membrane treated with 200 nM NE, and so did propranolol. Chlorpromazine did not inhibit MDA formation of NE-treated rbc membrane, but depressed slightly the MDA formation at 2-20 microM. The above-mentioned findings suggest that tricyclic antidepressants may increase dementia probably because of their enhancement of MDA formation in membrane lipids.
Keywords:
Tricyclic antidepressants amitriptyline (AMT) and imipramine (IMP) accelerated MDA formation at 20 nM-20 microM whenAMT and IMP was added to rbc membrane treated with 200 nM NE, and so did propranolol. Chlorpromazine did not inhibit MDA formation of NE-treated rbc membrane, but depressed slightly the MDA formation at 2-20 microM. The above-mentioned findings suggest that tricyclic antidepressants may increase dementia probably because of their enhancement of MDA formation in membrane lipids.