Effect of aclacinomycin B on DNA polymerase II of tumor cells
Abstract
DEAE-cellulose, phosphocellulose and DNA-agarose affinity chromatography were used to isolate and purify the DNA polymerase II from L1210 cells and Lewis lung carcinoma. The enzyme showed a single polypeptide band on the profile of non-denaturing polyacrylamide electrophoresis and possessed high sensitively to N-ethylmaleimide(NEM).Aclacinomycin B inhibited potently DNA polymerase II from the 2 sources. The IC50 was 3.6 microg/ 100 microL and 5.3 microg/100 microL for Lewis lung carcinoma and the L 1210 enzymes, repectively.
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