Original Articles

Anti-ophidic toxin action of Agkistrodon acutus serum

Jie-tang Huang, Xiao-lan Ren, Mei Zhang, Guo-qiang Teng


The lethal effect of the venom of was protected by its serum. The serum alleviated the tissue necrosis and protected the blood system of rabbits when given a lethal dose of the snake venom.
In mice the LD of snake serum>2.5 g/kg. In anesthetized dogs iv the snake serum 200 mg/kg caused no influence on respiration, blood pressure, ECG,peripheral blood picture, hepatic and renal functions. No pathologic changes were found after 7 d.
By ammonium sulfate fractional precipitation, DEAE-Sephadex A-50 column chromatography, CM-Celluloses C-32 column chromatography and polyacrylamide gel plate electrophoresis, an an-titoxin factor was purified from the snake serum. Its isoelectric pt=4.0 and its mol wt=c 70,000. The activity of this factor against venom was increased by 10 times.
Complement fixation tests and agar immunodiffusion tests proved that the antitoxic action of serum manifested no antigen-antibody reaction.

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