Original Articles

Immunofluorescent staining of myosin in cultured cardiomyopathic hamster heart cells.

Authors: Zeng-hong Tu, Larry F Lemanski

Abstract

Primary cultures of heart cells from normal and genetically cardiomyopathic (CM) hamsters were analyzed by immunofluorescent staining for myosin. Antimyosin specifically stains the myofibrillar A-bands. These immunofluorescent staining experiments demonstrate an abnormally disarrayed arrangement of myofibrils in CM cells when compared to normal; however, the staining of individual myofibrils is similar in CM and normal. Our results suggest that the aberrant pattern of myofibrils in CM cardiomyocytes result not from secondary effects of in vivo development,but more likely, from primary effects of the mutation.
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