Original Articles

Effect of ATP on cell agglutination of experimental tumors

Zu-wu Wang, Pei-qin Chou


ATP enhanced PHA-mediated cell agglutination of S180, ARS and HepA by 22, 15 and 20%, respectively (P<0.05). ATP inhibited ConA-mediated agglutination of HepA by 24%, but did not induce any significant effect upon WGA-mediated cell agglutination.
The metabolic inhibitors (DNP and NaN3) as well as several antitumor agents (10-hydroxycamptothecin, VCR, lycobetain, oxalysine and 8-Br-cAMP) showed no significant effects on lectin-mediated agglutination.
Both PHA-mediated cell agglutination and the enhancing effect of ATP disappeared whenthese two chemicals were entrapped by single layer lipid vesicles (SLV) which were prepared by sonication of phosphatidylcholine of soy bean.
PHA induced 67+/-(SD) 19% of agglutination of nuclei isolated from S180 by Triton X-100. But ATP exerted no effect on PHA-mediated nuclei agglutination.
It is surmised that, besides the receptor on plasmalemma, there are acceptors of PHA on nuclear envelope, whereas the effect of ATP occurs only on cell surface membrane.

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