Original Articles

Action of aluminum on high voltage-dependent calcium current and its modulation by ginkgolide B

Authors: Lei Chen, Chang-jin Liu, Ming Tang, Ai Li, Xin-wu Hu, Yi-mei Du, Jing-jing Shen, Yong-li Lu, Jurgen Heschler

Abstract

Aim: To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (IHVA) and its modulation by ginkgolide B (Gin B).
Methods: The whole-cell, patch-clamp technique was used to record IHVA from acutely isolated hippocampal CA1 pyramydal neurons in rats.
Results: Al 0.1 mmol/L (low concentration) reduced IHVA; Al 0.75 and 1.0 mmol/L (high concentrations) increased IHVA, and Al decreased and increased IHVA at intermediate concentrations of 0.25 and 0.5 mmol/L. The increase of IHVA by Al 1.0 mmol/L was enhanced by the adenylyl cyclase (AC) agonist forskolin and was partly abolished by the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) antagonist H-89, whereas the decrease observed with Al 0.1 mmol/L was neither reversed by forskolin nor affected by H-89. Gin B had no effect on IHVA in normal neurons, but canceled the increase in IHVA by 1.0 mmol/L Al.
Conclusion: The results indicate that the mechanism of Al affecting IHVA differs at different concentrations, and this may be attributed to its complex actions. Gin B could prevent neurons from injury by inhibiting calcium influx.
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