Baculovirus as a highly efficient expression vector in insect and mammalian cells
Abstract
Baculovirus has been widely used for the production of recombinant proteins in
insect cells. Since the finding that baculovirus can efficiently transduce mammalian
cells, the applications of baculovirus have been greatly expanded. The prospects
and drawbacks of baculovirus-mediated gene expression, either in insect or
in mammalian cells, are reviewed. Recent progresses in expanding the applications
to studies of gene regulation, viral vector preparation, in vivo and ex vivo gene
therapy studies, generation of vaccine vectors, etc are discussed and the efforts
directed towards overcoming the existing bottlenecks are particularly emphasized.
Keywords:
insect cells. Since the finding that baculovirus can efficiently transduce mammalian
cells, the applications of baculovirus have been greatly expanded. The prospects
and drawbacks of baculovirus-mediated gene expression, either in insect or
in mammalian cells, are reviewed. Recent progresses in expanding the applications
to studies of gene regulation, viral vector preparation, in vivo and ex vivo gene
therapy studies, generation of vaccine vectors, etc are discussed and the efforts
directed towards overcoming the existing bottlenecks are particularly emphasized.