Original Articles

Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells

Jing LIU, Qing-li WU, Yong-hong FENG, Yi-fu YANG, Xiao-yu LI, Jian-ping ZUO


Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from
Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expression
and interleukin-12 (IL-12) production from THP-1 cells.
Methods:THP-1 cells
were differentiated into macrophage-like cells by Me2SO, and then cultured with
IFN-γ (500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide.
The surface molecule expressions were analyzed on a FACScan flow cytometer.
IL-12p40, IL-12p70 were assayed by ELISA.
Results:Tripolide suppressed CD80
and CD86 expressions on IFN-γ (500 kU/L) and LPS (1 mg/L) activated THP-1 cells
at nontoxic dosages of 2.5–0.625 μg/L. Furthermore, the production of IL-12p40
and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide.
Conclusion: Triptolide impairs the antigen-presenting function by inhibiting CD80
and CD86 expressions and decreased IL-12p40 and IL-12p70 (bioactive form) productions
from the activated THP-1 cells.