Basic fibroblast growth factor protected forebrain against ischemia-reperfusion damage in rats
Abstract
AIM: To study the effect of basic fibroblast growth factor (bFGF) on acute forebrain ischemia-reperfusion injury in rats.
METHODS: Both vertebral arteries were occluded by electrocautery and severe but transient bilateral cerebral ischemia was produced by clamping both common carotid arteries for 20 min in rats. The contents of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in striatum were determined by HPLC with electrochemic detector (HPLC-EC) at 3, 6, 12, 24 h after the onset of reperfusion. The contents of sodium, potassium in forebrain were determined by atomic absorption spectro-photometric method at 6-h reperfusion. Morphological changes of the striatum were also examined.
RESULTS: At 6-h reperfusion, the DA content in striatum decreased from (99 +/- 16) micrograms.g-1 (protein) in sham-operation group to (70 +/- 20) micrograms.g-1 (protein); the water and the sodium contents in forebrain increased from 77.34% +/- 0.19% to 79.6% +/- 0.6% and from (9.3 +/- 0.6) to (10.5 +/- 0.6) mg.g-1 (dry weight), respectively. bFGF (i.v. 45 micrograms.kg-1.h-1 for 3 h) from the start of reperfusion prevented the decrease of DA in striatum and the increases of water and sodium contents in forebrain. Histological examination also indicated that bFGF ameliorated the injury of neurons.
CONCLUSION: bFGF prevented the brain from injury of ischemia and reperfusion.
Keywords:
METHODS: Both vertebral arteries were occluded by electrocautery and severe but transient bilateral cerebral ischemia was produced by clamping both common carotid arteries for 20 min in rats. The contents of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in striatum were determined by HPLC with electrochemic detector (HPLC-EC) at 3, 6, 12, 24 h after the onset of reperfusion. The contents of sodium, potassium in forebrain were determined by atomic absorption spectro-photometric method at 6-h reperfusion. Morphological changes of the striatum were also examined.
RESULTS: At 6-h reperfusion, the DA content in striatum decreased from (99 +/- 16) micrograms.g-1 (protein) in sham-operation group to (70 +/- 20) micrograms.g-1 (protein); the water and the sodium contents in forebrain increased from 77.34% +/- 0.19% to 79.6% +/- 0.6% and from (9.3 +/- 0.6) to (10.5 +/- 0.6) mg.g-1 (dry weight), respectively. bFGF (i.v. 45 micrograms.kg-1.h-1 for 3 h) from the start of reperfusion prevented the decrease of DA in striatum and the increases of water and sodium contents in forebrain. Histological examination also indicated that bFGF ameliorated the injury of neurons.
CONCLUSION: bFGF prevented the brain from injury of ischemia and reperfusion.