Original Article

Purification and anticancer activity of cytotoxin-14 from venom of Naja naja atra

Xiao-Yan Zhong, Guang-Feng Liu, Qing-Chuan Wang


Ion-chromatography of lyophilized cobra (Naja naja atra) venom on SP-Sephadex C-50 yielded 14 fractions, of which 7, 8, 9, and 10 possessed neurotoxic actions and 11, 12, 13, and 14 had cytotoxic activities. Chromatography of fraction 14 on SP-Sephadex C-25 gave cytotoxin-14. It was homogeneous on polyacrylamide gel electrophoresis with m = 7448 Da. It was a typical cytotoxin without phospholipase A2 activity and had a selective cytotoxic action on human cancer cell lines.

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