Original Article

1-Methyl-3-isobutylxanthine delays apoptosis induced by deprivation of growth factors in vascular endothelial cells

Authors: Jun-Ying Miao, Satohiko Araki, Shang-Li Zhang, Hiroshi Hayashi

Abstract

"AIM:
To investigate the effects of 1-methyl-3-isobutylxanthine (MIBX) on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells (VEC).
METHODS:
Nuclear fragmentation was observed by fluorescence microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was measured by agarose gel electrophoresis.
RESULTS:
The cells deprived of aFGF and serum were treated with MIBX 25-200 mumol/L for 3, 6, 9, and 12 h, respectively. Morphological changes including the formation of apoptotic bodies and DNA fragmentation of these cells were significantly suppressed by IBMX 50-200 mumol/L at 3 h and 6 h. But after 12-h treatment, no difference was observed between the treated and untreated cells.
CONCLUSION:
MIBX delays apoptosis of vascular endothelial cells induced by deprivation of aFGF and serum."
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