Distribution of functional P2X1-like receptor in isolated rabbit arteries.
Abstract
AIM: To clarify the distribution of functional P2X1-like receptors in rabbit
arteries.
METHODS: Isometric contractile responses to noradrenaline (NA) and alpha,
beta-methylene ATP (alpha, beta-meATP) were observed in the arteries isolated
from rabbit renal (Re), femoral (Fe), saphenous (Sa), mesenteric (Me), splenic
(Sp), and ear (Ea).
RESULTS: The maximal responses to NA (E(max.NA)) varied among the arteries in an
order of Re>Fe>Sa>Me>Sp>Ea. After standardization by the maximal response to KCl
(E(max.KCl)), however, the values of E(max.NA)/E(max.KCl) in the six kinds of
arteries were almost the same. EC50 values of NA in arteries were different, and
the EC50 value of NA in Me artery was 54 times that of Fe artery (P<0.01). The
maximal response to alpha, beta-meATP (E(max.alpha, beta-meATP) ) varied among
the arteries in an order of Re>Sa=Fe>Ea>Sp=Me, and the values of Emax.alpha,
beta-meATP /E(max.KCl) were still different (Fe
Keywords:
arteries.
METHODS: Isometric contractile responses to noradrenaline (NA) and alpha,
beta-methylene ATP (alpha, beta-meATP) were observed in the arteries isolated
from rabbit renal (Re), femoral (Fe), saphenous (Sa), mesenteric (Me), splenic
(Sp), and ear (Ea).
RESULTS: The maximal responses to NA (E(max.NA)) varied among the arteries in an
order of Re>Fe>Sa>Me>Sp>Ea. After standardization by the maximal response to KCl
(E(max.KCl)), however, the values of E(max.NA)/E(max.KCl) in the six kinds of
arteries were almost the same. EC50 values of NA in arteries were different, and
the EC50 value of NA in Me artery was 54 times that of Fe artery (P<0.01). The
maximal response to alpha, beta-meATP (E(max.alpha, beta-meATP) ) varied among
the arteries in an order of Re>Sa=Fe>Ea>Sp=Me, and the values of Emax.alpha,
beta-meATP /E(max.KCl) were still different (Fe