Original Article

GM-CSF and IFN-gamma-induced expression of human leucocyte antigen class II molecules on basophils of umbilical cord blood.

Authors: Mei-Lin XIE, Yin-Gen WU


AIM: To determine whether basophils expressed human leucocyte antigen (HLA) class
II molecules.
METHODS: Basophils in umbilical cord blood were separated and purified with
methods of density gradient centrifugation and immunomagnetic microbeads. The
isolated basophils were cultured in RPMI-1640 plus 10 % fetal calf serum at 37 in
a humidified atmosphere with 5 % CO2 and stimulated with granulocyte-macrophage
colony-stimulating factor (GM-CSF) or interferon (IFN)-gamma for 20-60 h. The
expression of HLA class II molecules on basophils was measured with fluorescence
activated cell sorter (FACS).
RESULTS: The purity of isolated basophils was > or = 83.5 %. After treated with
GM-CSF 10 microg/L or IFN-gamma 100 kU/L, the expression of HLA class II
molecules became detectable on membranous surface and at a higher level at 20 h,
the percentage of expression was 10.2 %+/-2.1 % and 11.3 %+/-1.0 %, respectively.
CONCLUSION: Basophils possessed the potency of HLA class II molecular expression.

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