Original Article

Effect of esculentoside A on autoimmunity in mice and its possible mechanisms.

Authors: Zhen-Yu XIAO, Qin-Yue ZHENG, Jun-Ping ZHANG, Yuan-Ying JIANG, Yang-Hua YI

Abstract

AIM: To investigate the influence of esculentoside A (EsA) on autoimmunity in
mice and its possible mechanisms.
METHODS: The level of anti-ds DNA antibody, proliferation of lymphoid cells, and
inflammation by pathologic section of joint in mice were examined. The
autoimmunity model is made through immunizing mice with formaldehyde treated
Campylobacter jejuni strain CJ-S131 and Freund's complete adjuvant. The apoptosis
of T cell was analyzed through morphology and flow cytometry (FACS). The
expression of ICAM-1 mRNA in human umbilical vein endothelial cell line (ECV304)
was determined by coupled reverse transcription and PCR amplification (RT-PCR).
RESULTS: EsA could potently lower the level of anti-ds DNA antibody, inhibit the
proliferation of lymphoid cells, and ameliorate inflammation in the joint of
model mouse. The apoptosis of thymocyte activated by ConA was markedly
accelerated while the expression of ICAM-1 mRNA in ECV304 was decreased by EsA.
CONCLUSION: EsA has the positive curative effect on autoimmunity in a mouse
model, which may function through inhibition of expression of ICAM-1 mRNA in
ECV304 and acceleration of thymocyte apoptosis.
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