Original Article

Inhibitory effects of Ginkgo biloba extract on vascular endothelial growth factor in rat aortic endothelial cells.

Authors: Li ZHANG, Yao-Cheng RUI, Peng-Yuan YANG, Yan QIU, Tie-Jun LI, Hou-Chun LIU

Abstract

AIM: To study the protective effects of Ginkgo biloba extract (GbE) against rat
aortic endothelial cells (RAEC) damage induced by lysophosphatidylcholine (LPC).
METHODS: Cell injury were determined by MTT assay and LDH release. Vascular
endothelial growth factor (VEGF) protein production from RAEC was determined by
enzyme-linked immunosorbent assay (ELISA). VEGF mRNA expression was examined by
in situ hybridization and dot blot.
RESULTS: GbE 0.01-1 microg/L prevented LPC-induced injury in cultured RAEC in a
concentration-dependent manner. Cultured RAEC could express VEGF protein and VEGF
mRNA was induced by LPC 5 mg/L. GbE could inhibit the expression of VEGF protein
and VEGF mRNA in co-cultured RAEC with LPC.
CONCLUSION: LPC could induce a strong expression of VEGF in RAEC. GbE could
protect RAEC against the LPC-induced damage and downregulate VEGF protein and
VEGF mRNA expression in cultured RAEC.
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