Low dose of resveratrol enhanced immune response of mice.
Abstract
AIM: To study the immune modulating effect of low dose of resveratrol.
METHODS: Concanavalin A (ConA) and Staphylococcus aureus Cowan (Sac) were used to
induce the activation of T lymphocyte and antigen presenting cell and cytokine
production. [3H]-Thymidine incorporation was used to evaluate the proliferation
of lymphocyte. Cytokine production was detected by ELISA method.
Dinitrofluorobenzene (DNFB) was used to induce mice delayed type hypersensitivity
(DTH, delayed hypersensitivity) response and ear swelling was used as an
evaluating indicator. Changes of lymphocyte subtypes were detected by flow
cytometry.
RESULTS: Resveratrol (0.75-6 micromol/L) concentration-dependently promoted
lymphocyte proliferation and IL-2 production induced by ConA. Sac induced IL-12
and IFN-gamma (interferon type II) production were also concentration-dependently
enhanced by resveratrol, while IL-10 production was inhibited. Resveratrol (4
mg/kg, ig) promoted DTH response of mouse, which was suppressed by ethanol (16 %,
w/v) consumption. Resveratrol treatment had no significant influence on
lymphocyte subtypes in mice, however it could reverse the suppressive effect of
ethanol both on macrophage percentage and on macrophage MHC-II molecule
expression.
CONCLUSION: Low dose resveratrol enhanced cell-mediate immune response. Promoting
Th1 cytokine production and influencing on macrophage function might be its
mechanisms.
Keywords:
METHODS: Concanavalin A (ConA) and Staphylococcus aureus Cowan (Sac) were used to
induce the activation of T lymphocyte and antigen presenting cell and cytokine
production. [3H]-Thymidine incorporation was used to evaluate the proliferation
of lymphocyte. Cytokine production was detected by ELISA method.
Dinitrofluorobenzene (DNFB) was used to induce mice delayed type hypersensitivity
(DTH, delayed hypersensitivity) response and ear swelling was used as an
evaluating indicator. Changes of lymphocyte subtypes were detected by flow
cytometry.
RESULTS: Resveratrol (0.75-6 micromol/L) concentration-dependently promoted
lymphocyte proliferation and IL-2 production induced by ConA. Sac induced IL-12
and IFN-gamma (interferon type II) production were also concentration-dependently
enhanced by resveratrol, while IL-10 production was inhibited. Resveratrol (4
mg/kg, ig) promoted DTH response of mouse, which was suppressed by ethanol (16 %,
w/v) consumption. Resveratrol treatment had no significant influence on
lymphocyte subtypes in mice, however it could reverse the suppressive effect of
ethanol both on macrophage percentage and on macrophage MHC-II molecule
expression.
CONCLUSION: Low dose resveratrol enhanced cell-mediate immune response. Promoting
Th1 cytokine production and influencing on macrophage function might be its
mechanisms.