Effects of cyclosporin A by aerosol on airway hyperresponsiveness and inflammation in guinea pigs.
Abstract
AIM: To study cyclosporin A (CsA) by aerosol for anti-asthmatic effects in guinea
pigs.
METHODS: PC200 changes of lung resistance (RL) in the antigen-challenged
sensitized guinea pig induced by acetylcholine (ACh) or histamine, and
eosinophils changes in bronchoalveolar lavage fluid (BALF) and pulmonary
histologic section induced by antigen in vivo in sensitized guinea pigs were
investigated.
RESULTS: Pretreatment with CsA 10 g/L and 20 g/L by aerosol but not with CsA 5
g/L, dexamethasone (DXM) 0.5 mg/kg by ip increased PC200 value and prevented ACh
or histamine-induced airway hyperresponsiveness. However, CsA 5 g/L also
prevented histamine-induced airway hyperresponsiveness. CsA 10 g/L, 20 g/L and
DXM 0.5 mg/kg reduced markedly eosinophil accumulation in BALF. The lymphocyte
accumulation induced by antigen was not changed significantly by CsA and DXM
tested. DXM 0.5 mg/kg increased number of neutrophil in the BALF. There was a
statistical significance comparison with CsA groups. In the pulmonary
histological studies, CsA 20 g/L and DXM 0.5 mg/kg also inhibited eosinophil
infiltration in the epithelium and subepithelial connective tissue of bronchi and
bronchioles.
CONCLUSION: Anti-inflammation and anti-hyperresponsiveness of CsA by aerosol in
animal model offered an experimental evidence for airway inhalation of CsA in the
treatment of asthma.
Keywords:
pigs.
METHODS: PC200 changes of lung resistance (RL) in the antigen-challenged
sensitized guinea pig induced by acetylcholine (ACh) or histamine, and
eosinophils changes in bronchoalveolar lavage fluid (BALF) and pulmonary
histologic section induced by antigen in vivo in sensitized guinea pigs were
investigated.
RESULTS: Pretreatment with CsA 10 g/L and 20 g/L by aerosol but not with CsA 5
g/L, dexamethasone (DXM) 0.5 mg/kg by ip increased PC200 value and prevented ACh
or histamine-induced airway hyperresponsiveness. However, CsA 5 g/L also
prevented histamine-induced airway hyperresponsiveness. CsA 10 g/L, 20 g/L and
DXM 0.5 mg/kg reduced markedly eosinophil accumulation in BALF. The lymphocyte
accumulation induced by antigen was not changed significantly by CsA and DXM
tested. DXM 0.5 mg/kg increased number of neutrophil in the BALF. There was a
statistical significance comparison with CsA groups. In the pulmonary
histological studies, CsA 20 g/L and DXM 0.5 mg/kg also inhibited eosinophil
infiltration in the epithelium and subepithelial connective tissue of bronchi and
bronchioles.
CONCLUSION: Anti-inflammation and anti-hyperresponsiveness of CsA by aerosol in
animal model offered an experimental evidence for airway inhalation of CsA in the
treatment of asthma.