Effects of hemodynamic changes on taurine release from posterior hypothalamus of freely moving rats
Abstract
AIM:
To study the effects of blood volume and vascular resistance on taurine release.
METHODS:
We used push-pull superfusion technique in the posterior hypothalamus of conscious freely moving rats. Taurine was determined in the superfusate by HPLC with fluorescence detection following automatic precolumn o-phthaldialdehyde (OPA) derivatization.
RESULTS:
Hypervolemia increased the release of taurine in the hypothalamus. Intravenous infusion of levarterenol (3 micrograms kg-1 min-1) elicited a pronounced pressor response and an increase in the release of taurine. Conversely, a controlled hemorrhagic hypotension or iv infusion of nitroprusside (30 micrograms kg-1 min-1) elicited a hypotension and a decrease in the release of taurine from the posterior hypothalamus.
CONCLUSION:
In the posterior hypothalamus, taurine might play an important role in central blood pressure regulation.
Keywords:
To study the effects of blood volume and vascular resistance on taurine release.
METHODS:
We used push-pull superfusion technique in the posterior hypothalamus of conscious freely moving rats. Taurine was determined in the superfusate by HPLC with fluorescence detection following automatic precolumn o-phthaldialdehyde (OPA) derivatization.
RESULTS:
Hypervolemia increased the release of taurine in the hypothalamus. Intravenous infusion of levarterenol (3 micrograms kg-1 min-1) elicited a pronounced pressor response and an increase in the release of taurine. Conversely, a controlled hemorrhagic hypotension or iv infusion of nitroprusside (30 micrograms kg-1 min-1) elicited a hypotension and a decrease in the release of taurine from the posterior hypothalamus.
CONCLUSION:
In the posterior hypothalamus, taurine might play an important role in central blood pressure regulation.