Tretinoin or retinol enhancement of lymphokine-activated killer cell proliferation and cytotoxicity against human bladder cancer cells in vitro
Abstract
AIM: To study the effect of tretinoin (Tre) or retinol (Ret) on the proliferation
of lymphokine-activated killer (LAK) cells in patients with transitional cell
cancer of bladder and their cytolysis to bladder tumor cells.
METHODS: LAK cell proliferation was assayed in the presence of either Tre or Ret
by cell counting. Human transitional bladder cancer cell lines BIU-87, EJ, or
bladder tumor cells (BTC) from patients with bladder cancer were used as target
cells and cytotoxicity of LAK cells was determined by
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
RESULTS: The proliferation of LAK cells induced by interleukin-2 (IL-2) was
stimulated by Tre or Ret (10-100 nmol.L-1). The cytotoxicity of LAK cells against
BIU-87, EJ cells, or BTC was enhanced by pretreatment of LAK cells with Tre or
Ret 10-100 nmol.L-1.
CONCLUSION: Tre or Ret enhances the proliferation and cytotoxicity of LAK cells
from patients with bladder cancer. Retinoids are potential in adoptive
immunotherapy of bladder cancer.
Keywords:
of lymphokine-activated killer (LAK) cells in patients with transitional cell
cancer of bladder and their cytolysis to bladder tumor cells.
METHODS: LAK cell proliferation was assayed in the presence of either Tre or Ret
by cell counting. Human transitional bladder cancer cell lines BIU-87, EJ, or
bladder tumor cells (BTC) from patients with bladder cancer were used as target
cells and cytotoxicity of LAK cells was determined by
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
RESULTS: The proliferation of LAK cells induced by interleukin-2 (IL-2) was
stimulated by Tre or Ret (10-100 nmol.L-1). The cytotoxicity of LAK cells against
BIU-87, EJ cells, or BTC was enhanced by pretreatment of LAK cells with Tre or
Ret 10-100 nmol.L-1.
CONCLUSION: Tre or Ret enhances the proliferation and cytotoxicity of LAK cells
from patients with bladder cancer. Retinoids are potential in adoptive
immunotherapy of bladder cancer.