Original Article

Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

Alina Elena Pârvu, Sandu Florin Alb, Alexandra Crăciun, Marian Aurel Taulescu
DOI: 10.1038/aps.2012.129


Alina Elena PÂRVU1, *, Sandu Florin ALB2, Alexandra CRĂCIUN3, Marian Aurel TAULESCU4
1Department of Pathophysiology, Faculty of Medicine, University of Medicine and Pharmacy “Iuliu Haţieganu,” Cluj-Napoca 400012, Romania; 2Department of Periodontology, Faculty of Medical Dentistry, University of Medicine and Pharmacy “Iuliu Haţieganum,” Cluj-Napoca 400012, Romania; 3Department of Biochemistry, Faculty of Medicine, University of Medicine and Pharmacy “Iuliu Haţieganu,” Cluj-Napoca 400012, Romania; 4Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural and Veterinary Sciences, Cluj-Napoca 400372, Romania

Aim: To evaluate the effectiveness of subantimicrobial-dose doxycycline (SDD) as an adjunct to scaling and root planing (SRP) treatment against the nitrosative stress of moderate to advanced chronic periodontitis.

Methods: Adults with untreated chronic periodontitis (n=174) were randomly administered SRP+SDD (n=87) (20 mg of doxycycline twice daily) or SRP+placebo (n=87) treatment for 3 months. At baseline and after 3 months, the probing depths (PD), bleeding on probing (BOP) and clinical attachment level (CAL) were measured, and a gingivomucosal biopsy was collected to assay the induction of nitric oxide synthase (iNOS) and 3-nitrotyrosine (3NT), and blood was collected to assay for total nitrites and nitrates (NOx) and 3NT.

Results: Compared to baseline, at the completion of treatment, significant decreases in the levels of tissue iNOS and 3NT and serum NOx and 3NT were observed in both groups. SRP+SDD yielded a greater reduction in the gingivomucosal and serum nitrosative stress markers than did SRP+placebo. PD, BOP, and CAL reduction were correlated with the nitrosative stress parameters.

Conclusion: On a short-term basis, SDD therapy may be used as an adjunct to SRP treatment against nitrosative stress in moderate to advanced chronic periodontitis.

Keywords: nitric oxide; nitrosative stress; chronic periodontitis; scaling and root planing treatment; doxycycline; stomatology

This work was financially supported by the Ministry of Education, Research and Youth, CNCSIS grant PNII-IDEI 1273/2008. We would like to thank Synevo Romania SRL for providing the blood samples and Mihaela BANCIU for editing the text.
* To whom correspondence should be addressed.
E-mail parvualinaelena@yahoo.com
Received 2012-04-09 Accepted 2012-08-06

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