Muscimol microinjection into cerebellar fastigial nucleus exacerbates stress-induced gastric mucosal damage in rats
Abstract
Jin-zhou ZHU1, 2, #, Su-juan FEI1, #, Jian-fu ZHANG1, 2, *, Sheng-ping ZHU1, 2, Zhang-bo LIU1, 2, Ting-ting LI1, 2, Xiao QIAO1, 2
1Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China; 2Department of Physiology, Xuzhou Medical College, Xuzhou 221002, China
Aim: To investigate the effects of microinjection of the GABAA receptor agonist muscimol into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage and the underlying mechanism in rats.
Methods: Stress-induced gastric mucosal damage was induced in adult male SD rats by restraining and immersing them in cold water for 3 h. GABAA receptor agonist or antagonist was microinjected into the lateral FN. The decussation of superior cerebellar peduncle (DSCP) was electrically destroyed and the lateral hypothalamic area (LHA) was chemically ablated by microinjection of kainic acid. The pathological changes in the gastric mucosa were evaluated using TUNEL staining, immunohistochemistry staining and Western blotting.
Results: Stress-induced gastric mucosal damage was induced in adult male SD rats by restraining and immersing them in cold water for 3 h. GABAA receptor agonist or antagonist was microinjected into the lateral FN. The decussation of superior cerebellar peduncle (DSCP) was electrically destroyed and the lateral hypothalamic area (LHA) was chemically ablated by microinjection of kainic acid. The pathological changes in the gastric mucosa were evaluated using TUNEL staining, immunohistochemistry staining and Western blotting.
Conclusion: Cerebellar FN participates in the regulation of stress-induced gastric mucosal damage, and cerebello-hypothalamic circuits contribute to the process.
Keywords: cerebellum; fastigial nucleus; decussation of superior cerebellar peduncle; lateral hypothalamic area; GABA; muscimol; bicuculline; gastric mucosa; stress-induced gastric mucosal damage; apoptosis
This project was supported by grants from the National Natural Science Foundation of China (No 30570671), the Educational Science Research Foundation of Jiangsu Province (No 10KJB310015), the Research Foundation of Xuzhou Medical College (Nos 07KJ58 and 07KJ34) and the Xuzhou Social Development Fund (No XM08C062).
# These authors contributed equally to this article.
* To whom correspondence should be addressed.
E-mail jfzhang@xzmc.edu.cn
Received 2012-05-15 Accepted 2012-09-29
Keywords:
1Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China; 2Department of Physiology, Xuzhou Medical College, Xuzhou 221002, China
Aim: To investigate the effects of microinjection of the GABAA receptor agonist muscimol into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage and the underlying mechanism in rats.
Methods: Stress-induced gastric mucosal damage was induced in adult male SD rats by restraining and immersing them in cold water for 3 h. GABAA receptor agonist or antagonist was microinjected into the lateral FN. The decussation of superior cerebellar peduncle (DSCP) was electrically destroyed and the lateral hypothalamic area (LHA) was chemically ablated by microinjection of kainic acid. The pathological changes in the gastric mucosa were evaluated using TUNEL staining, immunohistochemistry staining and Western blotting.
Results: Stress-induced gastric mucosal damage was induced in adult male SD rats by restraining and immersing them in cold water for 3 h. GABAA receptor agonist or antagonist was microinjected into the lateral FN. The decussation of superior cerebellar peduncle (DSCP) was electrically destroyed and the lateral hypothalamic area (LHA) was chemically ablated by microinjection of kainic acid. The pathological changes in the gastric mucosa were evaluated using TUNEL staining, immunohistochemistry staining and Western blotting.
Conclusion: Cerebellar FN participates in the regulation of stress-induced gastric mucosal damage, and cerebello-hypothalamic circuits contribute to the process.
Keywords: cerebellum; fastigial nucleus; decussation of superior cerebellar peduncle; lateral hypothalamic area; GABA; muscimol; bicuculline; gastric mucosa; stress-induced gastric mucosal damage; apoptosis
This project was supported by grants from the National Natural Science Foundation of China (No 30570671), the Educational Science Research Foundation of Jiangsu Province (No 10KJB310015), the Research Foundation of Xuzhou Medical College (Nos 07KJ58 and 07KJ34) and the Xuzhou Social Development Fund (No XM08C062).
# These authors contributed equally to this article.
* To whom correspondence should be addressed.
E-mail jfzhang@xzmc.edu.cn
Received 2012-05-15 Accepted 2012-09-29