Original Article

Suppression of channel conductance by diacetyl monoxime in guinea pig and embryonic chick cardiomyocytes

Hideaki Sada, Takashi Ban, Nicholas Sperelakis


AIM: To examine the effects of diacetyl monoxime (DAM), and putative dephospohrylating agent, on conductance of the cardiac Ca, Na, and K channels.
METHODS: The Ca (ICa), Na (INa), and K (IK) currents were recorded in single ventricular myocytes from guinea pigs and chick embryos before and after addition of DAM using the whole-cell voltage-clamp technique.
RESULTS: DAM 10 mmol.L-1 reduced rapidly the amplitudes of ICa (by about 30%), INa (by about 25%), and IK (by 25%-50%) without alterations of the voltage-dependence.
CONCLUSION: DAM was a channel inhibitor of the unique type having nonselective phosphatase activities.

Article Options

Download Citation

Cited times in Scopus