HPLC determination of nikethamide concentration in plasma of 6 patients with chronic obstructive pulmonary disease
Abstract
A rapid and quantitative analytical method for determination of nikethamide in human plasma was reported. High performance liquid chromatograph model KYS (Shanghai) fitted with a reversed-phase YWC C18H37 (10 μg) column and equipped with a 254 nm μv detector was used. The mobile phase consisted of 42% methanol, 58% 0.05 mol/L sodium acetate-acetic acid buffer (pH 4.5). Dapsonum was used as an internal standard. The retention time for nikethamide and internal standard were 8.75 and 6.00 min, respectively. The sensitivity was 10 ng or 2 μg/ml nikethamide in plasma. The within-day recovery rates and CV were 98.5±2.5 and 2.5%; and those of between-days were 96±4 and 3.7%, respectively.
Nikthamide was given by iv dipping at the rate of 18.4-18.8 mg/(kg•h) to 6 patients with COPD (chronic obstructive pulmonary disease). At the end of 2 and 4 h, the concentration of nikethamide were 62±17 and 119±42 μg/ml, respectively. The increase of the minute ventilation was 33±19 and 42±19%, respectively. Mild side-effects were seen when the nikethamide plasma concentration reached 90 μg/ml.
Keywords:
Nikthamide was given by iv dipping at the rate of 18.4-18.8 mg/(kg•h) to 6 patients with COPD (chronic obstructive pulmonary disease). At the end of 2 and 4 h, the concentration of nikethamide were 62±17 and 119±42 μg/ml, respectively. The increase of the minute ventilation was 33±19 and 42±19%, respectively. Mild side-effects were seen when the nikethamide plasma concentration reached 90 μg/ml.