Diethyldithiocarbamate inhibits iNOS expression in human lens epithelial cells stimulated by IFN-gamma and LPS
Abstract
Aim: To investigate the biological activity of human lens epithelial cells (HLEC)
in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and to
assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and
expression of NOS. Methods: The human lens epithelial cell line SRA 01/04 was
used in this experiment. Semi-quantitative reverse transcription polymerase chain
reaction (RT-PCR) and Western blotting were used to detect, respectively, iNOS
mRNA expression and protein production. Results: A costimulation by interferon
gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS expression
in HLEC. The expression of iNOS was significantly reduced in a dosedependent
manner by adding DDC from 10 μmol/L to 1 mmol/L. Conclusion:
The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and it
is inhibited by DDC.
Keywords:
in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and to
assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and
expression of NOS. Methods: The human lens epithelial cell line SRA 01/04 was
used in this experiment. Semi-quantitative reverse transcription polymerase chain
reaction (RT-PCR) and Western blotting were used to detect, respectively, iNOS
mRNA expression and protein production. Results: A costimulation by interferon
gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS expression
in HLEC. The expression of iNOS was significantly reduced in a dosedependent
manner by adding DDC from 10 μmol/L to 1 mmol/L. Conclusion:
The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and it
is inhibited by DDC.