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Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor

  
@article{APS9962,
	author = {Wei Tang and Ren Guo and Shi-jun Shen and Yang Zheng and Yu-ting Lu and Meng-meng Jiang and Xue Cui and Ci-zhong Jiang and Xin Xie},
	title = {Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor},
	journal = {Acta Pharmacologica Sinica},
	volume = {40},
	number = {5},
	year = {2019},
	keywords = {},
	abstract = {Human liver or hepatocyte transplantation is limited by a severe shortage of donor organs. Direct reprogramming of other adult cells into hepatic cells may offer a solution to this problem. In a previous study, we have generated hepatocyte-like cells from mouse fibroblasts using only one transcription factor (TF) plus a chemical cocktail. Here, we show that human urine-derived epithelial-like cells (hUCs) can also be transdifferentiated into human hepatocyte-like cells (hiHeps) using one TF (Foxa3, Hnf1α, or Hnf4α) plus the same chemical cocktail CRVPTD (C, CHIR99021; R, RepSox; V, VPA; P, Parnate; T, TTNPB; and D, Dznep). These hiHeps express multiple hepatocyte-specific genes and display functions characteristic of mature hepatocytes. With the introduction of the large T antigen, these hiHeps can be expanded in vitro and can restore liver function in mice with concanavalin-A-induced acute liver failure. Our study provides a strategy to generate functional hepatocyte-like cells from hUCs by using a single TF plus a chemical cocktail.},
	url = {http://www.chinaphar.com/article/view/9962}
}