TY - JOUR AU - VARGA Viktória AU - NÉMETH János AU - OLÁH Orsolya AU - TóTh-Szűki Valéria AU - kovácS Viktória AU - RemzSő Gábor AU - Domoki Ferenc PY - 2018 TI - Molecular hydrogen alleviates asphyxia-induced neuronal cyclooxygenase-2 expression in newborn pigs JF - Acta Pharmacologica Sinica; Vol 39, No 8 (August 2018): Acta Pharmacologica Sinica Y2 - 2018 KW - N2 - cyclooxygenase-2 (COX-2) has an established role in the pathogenesis of hypoxic-ischemic encephalopathy (hie). in this study we sought to determine whether COX-2 was induced by asphyxia in newborn pigs, and whether neuronal COX-2 levels were affected by H 2 treatment. Piglets were subjected to either 8 min of asphyxia or a more severe 20 min of asphyxia followed by H 2 treatment (inhaling room air containing 2.1% H 2 for 4 h). COX-2 immunohistochemistry was performed on brain samples from surviving piglets 24 h after asphyxia. The percentages of COX-2-immunopositive neurons were determined in cortical and subcortical areas. only in piglets with more severe hie, we observed significant, region-specific increases in neuronal COX-2 expression within the parietal and occipital cortices and in the cA3 hippocampal subfield. H 2 treatment essentially prevented the increases in COX-2-immunopositive neurons. in the parietal cortex, the attenuation of COX-2 induction was associated with reduced 8’-hydroxy-2’-deoxyguanozine immunoreactivity and retained microglial ramifcation index, which are markers of oxidative stress and neuroinflammation, respectively. This study demonstrates for the first time that asphyxia elevates neuronal COX-2 expression in a piglet hie model. Neuronal COX-2 induction may play region-specific roles in brain lesion progression during hie development, and inhibition of this response may contribute to the antioxidant/anti-inflammatory neuroprotective effects of H 2 treatment. UR - http://www.chinaphar.com/article/view/9826