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Transfer of the IL-37b gene elicits anti-tumor responses in mice bearing 4T1 breast cancer

   
@article{APS9424,
	author = {Wei-qiang WANG and Dan ZHAO and Yu-shan ZHOU and Xiao-yu HU and Zhi-na SUN and Gang YU and Wan-tong WU and Song CHEN and Jiu-long KUANG and Guo-gang XU and Zhong-chao HAN and Bang-mao WANG and Jing-xian YANG and Xiao-ming FENG},
	title = {Transfer of the IL-37b gene elicits anti-tumor responses in mice bearing 4T1 breast cancer},
	journal = {Acta Pharmacologica Sinica},
	volume = {36},
	number = {4},
	year = {2017},
	keywords = {},
	abstract = {Aim: IL-37b has shown anti-cancer activities in addition to its anti-inflammatory properties. In this study, we investigated the effects of IL-37b on breast carcinoma growth in mice and to determine the involvement of T cell activation in the effects.
Methods:  IL-37b gene was transferred into mouse breast carcinoma cell line 4T1 (4T1-IL37b cells), the expression of secretory IL-37b by the cells was detected, and the effects of IL-37b expression on the cell proliferation in vitro was evaluated. After injection of 4T1 cells or 4T1-IL37b cells into immunocompetent BALB/c mice, immunodeficient BALB/c nude mice and NOD-SCID mice, the tumor growth and survival rate were measured. The proliferation of T cells in vitro was also detected.
Results:  IL-37b was detected in the supernatants of 4T1-IL37b cells with a concentration of 12.02±0.875 ng/mL. IL-37b expression did not affect 4T1 cell proliferation in vitro. BALB/c mice inoculated with 4T1-IL37b cells showed significant retardation of tumor growth. BALB/c mice inoculated with both 4T1 cells and mitomycin C-treated 4T1-IL37b cells also showed significant retardation of tumor growth. But the anti-cancer activity of IL-37b was abrogated in BALB/c nude mice and NOD-SCID mice inoculated with 4T1-IL37b cells. Recombinant IL-37b slightly promoted CD4+ T cell proliferation without affecting CD8+ T cell proliferation.
Conclusion:  IL-37b exerts anti-4T1 breast carcinoma effects in vivo by modulating the tumor microenvironment and influencing T cell activation.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/9424}
}