TY - JOUR AU - LIAO Jia-yu AU - SONG Yang AU - LIU Yan PY - 2017 TI - A new trend to determine biochemical parameters by quantitative FRET assays JF - Acta Pharmacologica Sinica; Vol 36, No 12 (December 2015): Acta Pharmacologica Sinica Y2 - 2017 KW - N2 - Förster resonance energy transfer (FRET) has been widely used in biological and biomedical research because it can determine molecule or particle interactions within a range of 1–10 nm. The sensitivity and efficiency of FRET strongly depend on the distance between the FRET donor and acceptor. Historically, FRET assays have been used to quantitatively deduce molecular distances. However, another major potential application of the FRET assay has not been fully exploited, that is, the use of FRET signals to quantitatively describe molecular interactive events. In this review, we discuss the use of quantitative FRET assays for the determination of biochemical parameters, such as the protein interaction dissociation constant ( K d ), enzymatic velocity ( K cat ) and K m . We also describe fluorescent microscopy-based quantitative FRET assays for protein interaction affinity determination in cells as well as fluorimeter-based quantitative FRET assays for protein interaction and enzymatic parameter determination in solution. UR - http://www.chinaphar.com/article/view/9282