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A new trend to determine biochemical parameters by quantitative FRET assays

   
@article{APS9282,
	author = {Jia-yu LIAO and Yang SONG and Yan LIU},
	title = {A new trend to determine biochemical parameters by quantitative FRET assays},
	journal = {Acta Pharmacologica Sinica},
	volume = {36},
	number = {12},
	year = {2017},
	keywords = {},
	abstract = {Förster resonance energy transfer (FRET) has been widely used in biological and biomedical research because it can determine molecule or particle interactions within a range of 1–10 nm. The sensitivity and efficiency of FRET strongly depend on the distance between the FRET donor and acceptor. Historically, FRET assays have been used to quantitatively deduce molecular distances. However, another major potential application of the FRET assay has not been fully exploited, that is, the use of FRET signals to quantitatively describe molecular interactive events. In this review, we discuss the use of quantitative FRET assays for the determination of biochemical parameters, such as the protein interaction dissociation constant (Kd), enzymatic velocity (Kcat) and Km. We also describe fluorescent microscopy-based quantitative FRET assays for protein interaction affinity determination in cells as well as fluorimeter-based quantitative FRET assays for protein interaction and enzymatic parameter determination in solution.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/9282}
}