@article{APS9133,
author = {Xie-Chuan Weng and Xiao-Dan Gai and Jian-Quan Zheng and Jin Li},
title = {Agmatine blocked voltage-gated calcium channel in cultured rat hippocampal neurons},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the mechanism of agmatine by observing the effect of agmatine on the voltage-gated channels in rat hippocampal neurons.
METHODS: The whole-cell patch recording technique was performed to record the voltage-gated potassium, sodium, and calcium currents in cultured rat hippocampus. Agmatine was applied directly to the single neuron using a pressure injector with microtubules.
RESULTS: Agmatine (500 micromol/L) had no significant effect on the voltage-gated potassium and sodium channels. Agmatine reversibly blocked the voltage-gated calcium channel and the blockade was enhanced with the increasing concentration of agmatine. The inhibitory rates were 21%+/-4%, 35%+/-6%, 49%+/-6%, 67%+/-4%, 69%+/-6%, 86%+/-8%, and 87%+/- 9%, at the concentration of 0.1, 0.5, 1.0, 5.0, 10.0, 50.0, and 100 micromol/L, respectively. IC50 was (1.2+/-0.4) micromol/L. Two-way ANOVA revealed that change of membrane potential displayed a significant interaction with the blockade by agmatine.
CONCLUSION: Agmatine reversibly blocked the voltage-gated calcium channel in rat hippocampal neurons in a concentration- and voltage-dependent way. Agmatine might perform its physiological and pharmacological effects partially by blocking the calcium channel.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9133}
}