@article{APS8890,
author = {Xiao-Qiang Li and Ming-Gao Zhao and Qi-Bing Mei and Yan-Feng Zhang and Wei Cao and Hai-Fang Wang and Dan Chen and Yi Cui},
title = {Effects of tumor necrosis factor-alpha on calcium movement in rat ventricular myocytes},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {12},
year = {2016},
keywords = {},
abstract = {AIM: To study the effects of tumor necrosis factor-alpha (TNF-alpha) on calcium movement in rat ventricular myocytes.
METHODS: Intracellular free Ca2+ concentration was measured with calcium fluorescent probe Fluo-3/AM and laser confocal microscope. L-type calcium current (ICa,L) was recorded with the whole-cell configuration of the patch-clamp techniques.
RESULTS: At 2, 20 and 200 microg/L, TNF-alpha was found to increase intracellular free Ca2+ concentration in a dose-dependent manner illustrated by the increment of calcium fluorescence density with laser confocal microscope. Nicardipine 0.5 micromol/L slightly attenuated TNF-alpha-induced response. When the cardiac myocytes were exposed to caffeine (100 mmol/L) for 30 min, TNF-alpha failed to induce any change of intracellular free calcium. However, it was found that TNF-alpha inhibited I(Ca,L) in whole-cell patch-clamp experiments. At 2, 20, and 200 microg/L, TNF-alpha decreased peak I(Ca,L) by 3.9 % (-5.1 pA/pF+/-0.3 pA/pF vs -4.9 pA/pF+/-0.2 pA/pF, n=9, P>0.05), 15.7 % (-5.1 pA/pF+/-0.3 pA/pF vs -4.3 pA/pF+/-0.3 pA/pF, n=9, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8890}
}