@article{APS8861,
author = {Li-Wu Fu and Qi-Chao Pan and Wen-Long Huang and Xiao-Ping Yang},
title = {Reversal of tumor multidrug resistance by 2-phenyl-3-(3',5'-dimorpholinomethyl-4'-hydroxy)-benzoyl-indole (HWL-12)},
journal = {Acta Pharmacologica Sinica},
volume = {19},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: To explore the reversal of multidrug resistance (MDR) by indole derivative HWL-12.
METHODS: Cytotoxicity was determined by tetrazolium (MTT) assay. The function of P-gp was examined by Fura 2-AM assay. Cellular accumulation of doxorubicin (Dox) was measured by fluorescence spectrophotometry.
RESULTS: HWL-12 10 mumol.L-1 markedly increased Fura-2 accumulation and was 17.2-fold reversal of MDR in MCF-7/ADR cells. The cellular Dox accumulation in MDR cells was increased in the presence of HWL-12 on the MCF-7/ADR cells. No effect was observed for Dox accumulation in the presence of high Ca2+ (addition of CaCl2) or low Ca2+ (addition of egtazic acid).
CONCLUSION: HWL-12 has a potent MDR reversal action which was associated with the increase of cellular Dox accumulation in MDR cells and not related with calcium ion concentration.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8861}
}